Associate Scientist Job at Pacer Group, South San Francisco, CA

L0RReUQxK3NCNUN3Y29ZdGd3V1VqeVE0
  • Pacer Group
  • South San Francisco, CA

Job Description

Job Title: Scientist - II

Duration: 12 Months

Location: South San Francisco, CA 94080

Summary:

Education and experience required:

  • B.S. degree in Biochemistry, Cell/Molecular Biology, Bioprocess engineering or related fields and three (3) years of industry or academia experience or a M.S. degree with 1 year of experience in similar fields

Must haves:

  • Prior experience in mammalian cell culture techniques, and cell culture media preparation is a prerequisite: single use mammalian cell culture tools employed in bioprocess development, including but not limited to deep well plates and shake flasks.
  • Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).
  • Ability to multi-task different protocols at once, handle concurrent projects simultaneously with high attention to detail.

Nice to haves/preferred:

  • Experience with high-throughput systems and automation.
  • Experience with batch purification using a variety of scaffolds and basic QC.
  • Industry experience is preferred.

Software skills:

  • Basic knowledge on biological data collection and analysis tools (Vicell, metabolite analyzers) and computer applications such as ELN, MS Word, MS Excel and MS PowerPoint.
  • Experience with data and reagent management software such as Dotmatics, Benchling, Spotfire, etc is preferred.

Skills/techniques needed:

  • Excellent aseptic techniques and familiarity with principles of sterilization/disinfection.
  • Strong communication, organizational, and interpersonal skills.
  • Able to maintain a “can-do” attitude and act with a sense of urgency to complete innovative projects while being flexible in work priorities based on business needs.
  • Understand and adhere to corporate standards regarding code of conduct, safety, appropriate handling of materials
  • Able to troubleshoot cell culture system and think critically about experiments.
  • Must be comfortable with physical activities of constant standing, walking, and sitting with arm stretching into biosafety cabinets for cell culture work.

Responsibilities:

Department summary:

  • Our teams combine leading drug discovery capabilities and world-class R&D with the purpose of turning breakthrough science into life-changing medicines.
  • We are looking for a motivated scientist to be an integral part of the Protein Science department within Discovery Biologics at Client’s Research Labs- South San Francisco.
  • The successful candidate will be involved in multiple aspects of the production of recombinant proteins, monoclonal antibodies, multispecific scaffolds, and novel protein modalities to enable all aspects of Early Discovery.
  • Ideally, the candidate will be able to execute and develop on techniques involving recombinant protein expression in CHO cells using both transient and stable-pool techniques.
  • Additionally, the candidate should have foundational experience in purification and QC, along with a proven ability to accurately record and present data.

Job responsibilities in detail:

  • Maintain and propagate CHO cells in suspension culture independently to support ongoing projects in a cell culture lab.
  • Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293). Express and batch purify a variety of proteins (monoclonal, bispecific antibodies, VHHs, Fabs, Fc-Fusions, etc.) using both resin and magnetic-bead immunoprecipitation methods.
  • Execute fed-batch experiments in a variety of platforms: shake flasks 125ml to 5L, tube spins and multi-well plates.
  • Perform analytical measurements for cell growth, metabolite measurements and HPLC analysis of titers from supernatant.
  • Analyze purified proteins using techniques: SDS-PAGE, HPLC, UPLC, CESDS, Nano-drop, UV-Vis.
  • Maintain and troubleshoot mammalian cell culture systems.
  • Document protein production processes, prepare reports and communicate results
  • ELN documentation.

Key skills:

  • Maintain and propagate CHO cells in suspension culture independently
  • Perform transient and stable transfections for protein expression in mammalian cells(CHO cells).
  • Purification batch purification using bench top.
  • Candidate should have foundational experience in purification and QC

Job Tags

Flexible hours,

Similar Jobs

Alameda County Health

Management Analyst Job at Alameda County Health

 ...ALL County recruitments. MANAGEMENT ANALYST Alameda County Health, Housing and...  ...and deliver health care and services to people with Medi-Cal and without insurance, support...  ...perform all duties listed. 1. Plans analytical studies to be performed; defines and... 

PrismHR

Janitor Job at PrismHR

Title: Clean Tech Location: Greenwood Village, CO Pay: $20/hour Job Duties: Work in a team environment and follow directions from Supervisor and/or Team Lead Clean, chemically treat, and vacuum physical surfaces within a construction, technical or GMP environment...

ATC

Help Desk Support (Bilingual - Spanish) Job at ATC

 ...Help Desk Support (Bilingual - Spanish) Conyers, GA Hourly Pay: $22/hour on a 1099 basis Job Description: MUST be Bilingual, Spanish/English Provides technical assistance to both internal and external customers for tier one issues. This position requires... 

Robert Half

Bank Teller Job at Robert Half

Description We are looking for an experienced Bank Teller to join our team in San Diego, California. This is a long-term contract (90...  ...customers with information and guidance. Requirements - Previous experience as a Bank Teller or extensive cash handling experience is... 

Ultimate Staffing

Bilingual Administrative Assistant Job at Ultimate Staffing

 ...Administrative Assistant to support day-to-day office operations in our client's Bridgeport location. This role requires fluency in both English and Spanish to effectively communicate with internal teams and a diverse client or customer base. The ideal candidate will be...